FIELD: medicine; veterinary science.
SUBSTANCE: method of antigen production includes passage of agent Anaplasma marginale for susceptible cattle, cattle splenectomy followed by infection with infected Anaplasma marginale blood, exsanguination of infected animal and blood mixing with anticoagulant, centrifuge separation of plasma from erythrocytes, erythrocyte washing to remove remained plasma with physiologic saline, erythrocyte destruction, centrifuge removal of destroyed erythrocyte stroma and leukocytes. Thus centrifuge separation of plasma from erythrocytes is carried out at 3000 - 3500 rpm within 10-15 minutes. Erythrocyte washing to remove remained plasma in physiologic saline is triple centrifugation at the same modes. Erythrocyte destruction is carried out through triple freezing and defrosting. Produced lysed erythrocytes with released Anaplasmas are mixed with tenfold amount of sterile physiologic saline. Removal destroyed erythrocyte stroma and leukocytes is performed through triple centrifugation at g=5000-6000 during 45-60 min to have pure deposit Anaplasma marginale. Produced deposit is added with sterile physiologic saline. Mixture is shuttled within 3-6 hours, filtered, and anaplasma antigen is produced. Method of vaccine preparation includes suspending of produced antigen in concentration 55-75 billion anaplasmas in 1 ml with protein content 12-15 mg/ml in adjuvant. Produced suspension is additionally added with antibiotic. Vaccine contains produced antigen in amount 40-51.7 wt % and antibiotic. Animals are vaccinated with prepared vaccine hypodermically in dose 1-2 ml/gol or intradermally in dose 0.2 ml/gol twice every 30-45 days.
EFFECT: ecologically safe inactivated stable emulsified vaccine provides long and intense immunity.
17 cl, 3 ex
