METHOD OF HISTOLOGICAL PREPARATION STAINING Russian patent published in 2003 - IPC

FIELD: medicine, histology. SUBSTANCE: method involves running material by histological battery and pouring in paraffin followed by replacing to blocks. Serial histological slices are prepared using microtome and applied their on slides. After deparaffinization in xylene preparations are replaced firstly in 95^° and then in 70^° alcohol followed by rinsing in 2 portions of distilled water. Two drops of 2% solution of blue strong BB are applied on slices. In 5 min solution is poured off, 2 drops of the same solution and 2 drops of dye are applied (dye composition: 0.6 g of methyl green and 0.4 g of pyronine G are mixed in 20 ml of pharmaceutical glycerol and 80 ml of distilled water). Slice is stained for 5 min, rinsed with 2 portions of distilled water and dried on filter paper. For differentiation of nuclei 2 changes of 96^° ethyl alcohol are used. Preparation are placed into Canadian balsam with preliminary clearing in weighing bottle with meta-xylene for 5 min. Method provides stable staining cells and cellular structures, ensures to correct intensity staining of different structures without loss of quality of histological preparations. Invention can be used in morphological investigations in pathological anatomy, cytology and forensic medicine. EFFECT: improved method of staining.