METHOD FOR STAINING NUCLEOLAR ORGANISERS ON HISTOLOGICAL PREPARATIONS AND CYTOLOGICAL SMEARS Russian patent published in 2012 - IPC G01N33/48 G01N1/30 

FIELD: medicine.

SUBSTANCE: paraffin sections are prepared, fixed, coated with a colloidal developer prepared by mixing 2% aqueous gelatin in 1% formic acid and 50% aqueous AgNO₃ in equal proportions, incubated for 30-60 minutes, washed and contrasted. The material is fixed in 10% neutral buffered formalin (pH 7.4) for 24 hours. The material is finished in ascending alcohols, encapsulated in paraffin to prepare the sections which are prepared in 2% formic acid in 96% ethanol for 20 minutes, dehydrated in 2 portions of 96%; the sections are prepared in 0.1% NaOH for 2 min 30 sec. Then they are dried. One drop of 100% AgNO₃ is layered on the preparation. It is followed by incubation in a thermostat at 60°C for 1 min 40 sec in a humid chamber, and coating with one drop of 40% formaldehyde and colloidal developer. It is incubated for 20-50 seconds in the thermostat at the same temperature. The sections are washed in 4 portions of distilled water. The sections are processed with acetate buffer pH 2.4 for 1 minute; the preparations are contrasted with 0.2% aqueous methyl green for 10-15 seconds, purified in chloroform, differentiated for 2-3 minutes in n-butyl alcohol, processed in toluene and encapsulated in polystyrene.

EFFECT: higher quality of detection and evaluation of nucleolar organisers.

1 ex, 2 dwg