FIELD: microbiology, dairy industry. SUBSTANCE: sample is mixed with mixture of polymyxin M-sulfate in the concentration 250 mcg/ml and trimethoprim in the concentration 100-120 mcg/ml taken in equal ratio, grown additionally for 4-6 h at 37 C and centrifuged at 5000- -5500 rev/min for 30-40 min. Culturing upper layer of grown additionally sample is carried out in differential-diagnostic medium at 37 C for 18-20 h and number of grown colonies is recorded in ammonia vapor. Method provides enhancement of reliability of anthrax pathogen detection in milk. Invention can be used in laboratory analysis of milk. EFFECT: improved method of detection. 2 tbl, 2 ex
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