METHOD FOR DETECTION OF CLINICALLY RELEVANT ALTERNATIONS OF ONCOGENE KI-RAS DEOXYRIBONUCLEIC ACID SEQUENCE, ITS APPLICATION AND SET FOR EARLY TUMOR DETECTION TESTING Russian patent published in 2003 - IPC

FIELD: medicine, oncology, biochemistry. SUBSTANCE: invention relates to method for detection of clinically relevant alternations of oncogene Ki-ras deoxyribonucleic acid sequence, its application and set for early tumor detection testing. Method involves extraction of genome deoxyribonucleic acid from clinical samples by multiple steps of purification and removal of interference substances (inhibitors) and detection of alternations by complementary reaction of base hybridization after addition of six oligonucleotides with the defined complementary to the alternated genome DNA sites sequence. The set for carrying out the test for early tumor based on clinically relevant alternations of oncogene Ki-ras DNA sequence comprises the corresponding reagents and oligonucleotide probes. EFFECT: improved method of detection, enhanced sensitivity of method. 20 cl