APPLICATION OF A T5 PROMOTER FOR THE EXPRESSION OF THE GENE OF BETA-LYTIC PROTEASE LYSOBACTER CAPSICI VKM B-2533 AND STRAIN L. CAPSICI BLP P BEING PRODUCER OF SAID PROTEASE Russian patent published in 2023 - IPC C12N1/21 C12N9/52 C12N15/57 C12N15/63 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, namely, to the production of beta-lytic protease Blp. Proposed is a plasmid DNA pBBR1-MCS5blp-P_T5 ensuring the expression of beta-lytic protease Blp and containing the nucleotide sequence SEQ ID NO: 1 including the sequence of the T5 promoter, the blp gene encoding Blp from L. capsici VKM B-2533^T, and the sequence of the lambda t₀ transcription terminator. Also proposed is a strain L. capsici blp P_T5 being a producer of beta-lytic protease Blp, produced through the transformation of the strain L. capsici VKM B-2533^T with plasmid DNA pBBR1-MCS5blp-P_T5.

EFFECT: possibility of producing Blp with an output of 43.99±3.79 mg/l.

2 cl, 1 dwg, 2 ex