FIELD: veterinary virology, biotechnology. SUBSTANCE: virus vaccine contains virus-containing liquid (VL) out of attenuated "VGNKI-4" strain of homologous virus and drying medium (DM) in efficient ratio. Strain is deposited in VGNKI collection under registration name "VGNKI-4" DEP. Virus is reproduced both in monolayer and suspension cellular VNK-21 culture. Strain is applied at infectious activity to be not more than 6.0 Ig TCD50/cm. Obtained VL is mixed with DM. Mixture obtained is poured into vials per 2-4 cu. cm to undergo freeze drying. Vaccine should be applied twice at interval of 14-21 d at the volume of 1 cu. cm. Vaccinating dosage is not less than 105,0TCD50 PI-3 virus. After repeated vaccination immunity lasts for not less than 6 mo. EFFECT: higher efficiency. 2 cl, 6 ex, 1 tbl
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Authors
Dates
2003-09-27—Published
2001-11-08—Filed