METHOD FOR DETECTING ANTIBIOTICS CONTAINING β-LACTAM CYCLE IN LIQUID OF BIOLOGICAL ORIGIN AND ANALYTICAL KIT FOR ITS IMPLEMENTATION Russian patent published in 2003 - IPC

FIELD: medicine. SUBSTANCE: the present innovation deals with techniques to analyze biological liquids and includes the following stages: a) contact of certain volume of mentioned biological liquid with some amount of recognizing agent and thermostating of the mixture obtained under conditions when complex of antibiotics is developed which, probably, are present in biological liquid mentioned, together with recognizing agent; b) contact of the mixture developed at a)-stage with, at least, one standard antibiotic immobilized at support under conditions of development of the complex of standard antibiotic with that quantity of recognizing agent which did not react at a)-stage; and b) detection of the quantity of recognizing agent fixed at the support. Quantity of antibiotic is determined by that of recognizing agent. The latter contains receptor sensitive to antibiotics with betalactam cycle obtained out of Bacillus licheniformis. Analytical kit to detect antibiotics contains recognizing agent sensitive to antibiotics containing β-lactam cycle obtained out of Bacillus licheniformis and standard antibiotic immobilized upon support. The present innovation enables to shorten and simplify technique for analysis and increase sensitivity in detecting wide spectrum of antibiotics. EFFECT: higher efficiency of detection. 13 cl, 1 dwg