FIELD: biotechnology.
SUBSTANCE: disclosed is a method for microscopic analysis of a cell sample. Method comprises pre-incubation of a sample with a suspension of carbon quantum dots conjugated with an antibody against a molecular marker in the sample, and analysing the obtained sample using fluorescence microscopy by detecting a fluorescent signal of carbon quantum dots as a measure of the presence of a molecular marker in the cell sample. Carbon quantum dots are conjugated with an antibody against a molecular marker by planting said antibody on the surface of carbon quantum dots by adding a salt to a solution containing said antibody and a suspension of carbon quantum dots.
EFFECT: invention provides a method for microscopic analysis of a cell sample using fluorescent nanoagents, more readily available in production, not subject to photodegradation effects, having smaller dimensions and allowing their easy conjugation with biological receptors recognizing the desired targets in a microscopic sample.
19 cl, 8 dwg, 23 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING AN ANALYTE IN A SAMPLE USING IMMUNOCHROMATOGRAPHY | 2023 |
|
RU2835200C1 |
| METHOD FOR CYTOMETRIC ANALYSIS OF A SAMPLE | 2023 |
|
RU2835213C1 |
| METHOD OF SYNTHESIS OF FUNCTIONAL CARBON QUANTUM DOTS | 2023 |
|
RU2824005C1 |
| METHOD OF DELIVERING NUCLEIC ACID INTO A CELL | 2023 |
|
RU2835201C1 |
| METHOD OF SOLID-PHASE CATALYTIC ANALYSIS OF ANALYTE CONTENT IN SAMPLE USING SILVER NANOWIRES | 2022 |
|
RU2808559C1 |
| METHOD OF MEASURING pH BASED ON SILVER NANOWIRES | 2022 |
|
RU2808717C1 |
| METHOD FOR CATALYTIC DECOMPOSITION OF HYDROGEN PEROXIDE USING SILVER NANOWIRES | 2022 |
|
RU2815436C1 |
| FLUORESCENT MULTI-PURPOSE NANOSCALE LABEL AND CONJUGATES BASED THEREON | 2021 |
|
RU2777648C1 |
| CARBENE LIGAND AND ULTRASTABLE GOLD NANOPARTICLES ON ITS BASIS | 2021 |
|
RU2792581C1 |
| METHOD FOR PRODUCING CONJUGATE FOR PERFORMING STEREOSPECIFIC ANALYSIS | 2005 |
|
RU2314827C2 |
