FIELD: biotechnology, biochemistry. SUBSTANCE: binding modulating agent between polypeptide-αd (αd-subunit of α-integrin) and VCAM-1 are identified by contact of polypeptide-β2- with amino acids sequence SEQ ID N2, 53, 55 or 103 and VCAM-1 and by detection of binding. Polypeptide-αd or VCAM-1 are immobilized on a solid carrier and non-immobilized binding partner is labeled with detectable agent, in part, fluorescent agent. Binding detection is carried out by light emission from fluorescent agent. Invention allows the development of practical agents for therapeutic interferences with immune and inflammatory responses associated with αd-integrin. Invention can be used for search of modulating agent of biological activity of human β2-integrin. EFFECT: improved identifying method. 4 cl, 4 dwg, 2 tbl, 37 ex _
