FIELD: protein engineering, biochemistry. SUBSTANCE: amino acid residues of enzyme contacting with substrate into the binding channel are identified and then replaced for another residues that have the same charge indices and similar size of by-side chain. The replacement in acyl-ACP desaturase at positions 114, 115, 117, 118, 179, 181, 188 and 189 results to the alteration of the chain length and specificity in arrangement of double bonds in enzyme that changes its activity. EFFECT: valuable biochemical properties of modified enzyme. 24 cl, 1 dwg, 2 ex
Title |
Year |
Author |
Number |
GENES ENCODING DESATURASES AND THEIR APPLYING |
1998 |
|
RU2248397C2 |
FATTY ACID MIXTURES AND USE THEREOF |
2007 |
- Knut Mark I.
- Bitehm Piter R.
- Uolker Kejt A.
- Gogehl Gregori Frehnsis Uill'Jam
|
RU2483057C2 |
DELTA-8 DESATURASE GENES, ENZYMES ENCODED THEREBY AND USE THEREOF |
2009 |
- Das Tapas
- Mukerdzhi Pradip
- Perejra Sjuzett
- Krishnan Padmavati
|
RU2517615C2 |
DELTA 6-ACETYLENASE/DELTA 6-DESATURASE AND DELTA 6-DESATURASE FROM Ceratodon purpureus |
2000 |
- Khajnts Ehrnst
- Stimne Sten
- Li Mikhaehl
- Girke Tomas
- Shperling Petra
- Zaehringer Ul'Rikh
|
RU2268939C2 |
NOVEL ELONGASE-ENCODING GENE AND METHOD FOR PREPARING POLYUNSATURATED ALIPHATIC ACIDS |
2001 |
- Khajnts Ehrnst
- Tsank Torsten
- Tseringer Ul'Rikh
- Lerkhl' Jens
- Rents Andreas
|
RU2311457C2 |
DNA FRAGMENT (VARIANTS), METHOD OF PLANT PREPARING, METHOD OF INDUCTION OF GAMMA-LINOLENIC ACID PRODUCING (VARIANTS), METHOD OF INDUCTION OF OCTADECATETRAENOIC ACID AND DELTA-6-DESATURASE PRODUCING IN CYANOBACTERIUM |
1992 |
- Tomas Terri L.
- Reddi Avutu S.
- Nassio Majkl
- Frejssen Zhorzh L.
|
RU2152996C2 |
POLYNUCLEOTIDE, CODING ACYL-COA-SYNTHETASE HOMOLOGUE AND ITS APPLICATION |
2011 |
|
RU2528248C2 |
METHOD OF PRODUCING LIPIDS |
2012 |
- Vanerke Tomas
- Petri Dzhejms Robertson
- El Takhchi Anna
- Singkh Surinder Pal
- Lyu Tsin
|
RU2656996C2 |
GENE ENCODING ALIPHATIC ACID SERIES DESATURASE FROM PLANTS |
2000 |
- Fojssner Ivo
- Khornung Ehllen
- Fritshe Katrin
- Pajtssh Nikola
- Rents Andreas
|
RU2274657C2 |
BIOLOGICALLY ACTIVE ANIMAL FATTY ACID Δ-DESATURASE, DNA SEQUENCE ENCODING THE SAME, CLONING END EXPRESSING VECTORS CONTAINING SAID SEQUENCE, METHOD FOR PRODUCTION OF POLYUNSATURATED FATTY ACIDS, METHOD FOR CONVERTING OF DIHOMO-γ-LINOLENIC ACID TO ARACHIDONIC ACID, PROBE (VARIANTS), AND METHOD FOR DETECTION OF Δ-DESATURASE USING THE SAME |
1998 |
- Nejpir Dzhonatan A.
- Majklson Luiza
- Stoubart Kit
|
RU2266330C2 |