BIOLOGICALLY ACTIVE ANIMAL FATTY ACID Δ-DESATURASE, DNA SEQUENCE ENCODING THE SAME, CLONING END EXPRESSING VECTORS CONTAINING SAID SEQUENCE, METHOD FOR PRODUCTION OF POLYUNSATURATED FATTY ACIDS, METHOD FOR CONVERTING OF DIHOMO-γ-LINOLENIC ACID TO ARACHIDONIC ACID, PROBE (VARIANTS), AND METHOD FOR DETECTION OF Δ-DESATURASE USING THE SAME Russian patent published in 2005 - IPC

Abstract RU 2266330 C2

FIELD: biotechnology, in particular gene engineering, pharmaceutical and food processing industry.

SUBSTANCE: DNA sequence (1341 n.p.) encoding fatty acid -desaturase (447 amino acid residue, 57 kD) of nematode Caenorhabditis elegants is isolated and characterized. Obtained DNA-sequence is expressed in bacterium and yeast cells to produce Biologically active enzyme recombinant form. Said recombinant form is capable to catalyze conversion of dihomo-γ-linolenic acid to arachidonic acid and eicosatetraenoate to eicosapentaenoate.

EFFECT: method for large-scale production of polyunsaturated fatty acid.

15 cl, 4 dwg, 1 ex

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RU 2 266 330 C2

Authors

Nejpir Dzhonatan A.

Majklson Luiza

Stoubart Kit

Dates

2005-12-20—Published

1998-12-23—Filed