METHOD FOR OBTAINING LOW-MOLECULAR FRACTION OUT OF PORCINE PLACENTAL TISSUE Russian patent published in 2004 - IPC

FIELD: veterinary science, medicine, cosmetology. SUBSTANCE: the suggested method deals with preliminary washing out placental tissues in water, reducing and isolating a water-soluble composition, moreover, after washing out is over one should carry out rapid tissue freezing up to - 80 - -120 C with liquid nitrogen vapors. Reduction should be conducted in cryogenic mills at -80 C -120 C up to particles size being 20-30 mcm followed by separation of low-molecular fraction in vacuum chambers at the pressure of 0.1 mm mercury column and -10 C - -20 C with subsequent condensation onto cryogenic panels cooled with liquid nitrogen. The method enables to obtain highly efficient biologically active fraction with molecules having native structure at the weight being 300-350 atomic units of weight, not more. EFFECT: higher efficiency. 1 ex, 2 tbl