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Application

2002129989/13, 2002-11-12

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Start date

2002-11-12

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Actual filing date

2002-11-12

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Published

2004-05-27

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Inventor

Beljakov N.V.Vardanjan N.V.Denisov L.A.Zerov Ju.P.Korobitsyn L.P.Kalinin Ju.T.Murashov B.V.Pivovarov A.M.Popov O.V.Prokop'Ev A.A.

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Holder

Obshchestvo S Ogranichennoj Otvetstvennost'Ju Kontur" Sankt-Peterburg, Ul. Pudozhskaja,

RECOMBINANT PLASMID DNA pZIFN2α ENCODING SYNTHESIS OF HUMAN ALPHA-2b-INTERFERON AND STRAIN ESCHERICHIA COLI AS PRODUCER OF HUMAN ALPHA-2b-INTERFERON Russian patent published in 2004 - IPC

Abstract RU 2229517 C1

FIELD: biotechnology, molecular biology, protein engineering. SUBSTANCE: invention relates to technology for preparing recombinant proteins and can be used for preparing human recombinant alpha-2b-interferon. Recombinant plasmid DNA pZIFN2α encoding synthesis of human alpha-2b-interferon with the size 3337 base pairs comprises DNA fragment Nde I-Not I with the size 525 base pairs involving nucleotide sequence encoding human alpha-2b-interferon, DNA fragment Not I-Nde I of plasmid pLEX with the size 2812 base pairs. The strain of bacterium Escherichia coli ZIFN2α is a producer of human recombinant alpha-2b-interferon is prepared by transformation of microorganisms Escherichia coli with plasmid pZIFN2α. Invention provides preparing highly productive strain of Escherichia coli ZIFN2α that can be used for industrial synthesis of human alpha-2b-interferon. EFFECT: valuable properties of strain. 3 cl, 3 dwg, 1 tbl, 3 ex

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RU 2 229 517 C1

Authors

Beljakov N.V.

Vardanjan N.V.

Denisov L.A.

Zerov Ju.P.

Korobitsyn L.P.

Kalinin Ju.T.

Murashov B.V.

Pivovarov A.M.

Popov O.V.

Prokop'Ev A.A.

Dates

2004-05-27—Published

2002-11-12—Filed