PLASMID VECTOR PRH15A FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B, BACTERIAL STRAIN ESCHERICHIA COLI BL21 DE3 - PRODUCER OF METHIONINE-FREE INTERFERON ALPHA-2B AND METHOD FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B Russian patent published in 2019 - IPC C12N15/21 C12N15/57 C12N15/63 C12N15/70 C12N1/21 C12R1/19 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology. Disclosed are plasmid expression vector pRh15A for producing human methionine interferon alpha-2b, a human-producing derivative of human methionine interferon alpha-2b and a method for producing human methionine-free interferon alpha-2b. Plasmid expression vector pRh15A contains a gene of methionine aminopeptidase Escherichia coli, a rhamnose promoter (pBADrha), a rhaBAD operon, a terminator of transcription of phage T7 genes, origin of plasmid replication p15A, chloramphenicol acetyltransferase gene (CamR) and restriction sites required for combining separate fragments in a vector. Presented strain is Escherichia coli strain BL21Star [DE3] containing a plasmid expression vector pAYC-ET-(hIFN-a2b)-lacI and vector pRh15A. Method for producing methionine-free human interferon alpha-2b, involving culturing said strain, separating interferon-containing biomass followed by chromatographic purification of the end product.

EFFECT: group of inventions provides producing completely methionine-free interferon alpha-2b with high output.

3 cl, 4 dwg, 4 ex