Show metadata Hide metadata

(19)

(11)

2 326 168

(13)

(51)

IPC

C12N15/21(2006-01-01)

C12N15/63(2006-01-01)

C12N1/21(2006-01-01)

C12R1/19(2006-01-01)

(21) (22)

Application

2006137144/13, 2006-10-20

(24)

Start date

2006-10-20

(22)

Actual filing date

2006-10-20

(45)

Published

2008-06-10

(72)

Inventor

Shingarova Ljudmila NikolaevnaBoldyreva Elena FilippovnaNekrasova Oksana Vasil'EvnaGoncharova Ol'Ga VladimirovnaChugunova Nadezhda MuldabekovnaChugunov Aleksandr MikhajlovichEfanov Jurij Georgievich

(73)

Holder

Zakrytoe Aktsionernoe Obshchestvo

RECOMBINANT PLASMID DNA pIF TREN CODING HUMAN POLYPEPTIDE OF ALFA-2b INTERFERON AND POLYPEPTIDE OF Escherichia BACTERIA RACE coli-PRODUCER OF HUMAN ALFA-2b INTERFERON Russian patent published in 2008 - IPC C12N15/21 C12N15/63 C12N1/21 C12R1/19

Abstract RU 2326168 C1

FIELD: gene engineering.

SUBSTANCE: invention relates to gene engineering and may be used for production of recombinant polypeptide of human alfa-2b interferon. Recombinant plasmid DNA is constructed in vitro. It includes synthetic gene of human alfa-2b interferon, tandem of A2 and A3 promoters from the early stage of bacteriophage T7 and synthetic section - translation enhancer. All these elements determine constitutive biosynthesis of target protein in transformed by this DNA race of Escherichia coli SGK25 /pIF TREN with high yield.

EFFECT: high yield.

2 cl, 5 dwg, 5 ex

Similar patents RU2326168C1

Title	Year	Author	Number
RECOMBINANT PLAZMIDNAJA DNA pTrcIFdL CODING POLYPEPTIDE WITH HUMAN GAMMA INTERFERON ACTIVITY AND Escherichia coli BACTERIA STRAIN - PRODUCER OF POLYPEPTIDE WITH HUMAN GAMMA INTERFERON ACTIVITY	2009	Shingarova Ljudmila Nikolaevna Boldyreva Elena Filippovna Tikhonov Roman Vladimirovich Jakimov Sergej Aleksandrovich Vul'Fson Andrej Nikolaevich Dolgikh Dmitrij Aleksandrovich Kirpichnikov Mikhail Petrovich	RU2399670C1
RECOMBINANT PLASMID DNA pA3GF CODING POLYPEPTIDE OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR, AND Escherichia BACTERIA RACE coli-PRODUCER OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR POLYPEPTIDE	2006	Shingarova Ljudmila Nikolaevna Boldyreva Elena Filippovna Petrovskaja Lada Evgen'Evna Pustoshilova Nina Mikhajlovna Goncharova Ol'Ga Vladimirovna Chugunova Nadezhda Muldabekovna Chugunov Aleksandr Mikhajlovich Efanov Jurij Georgievich	RU2326169C1
RECOMBINANT PLASMID DNA PES4-4 ENCODING HUMAN POLYPEPTIDE INTERFERON ALPHA-2B AND STRAIN ESCHERICHIA COLI BREES4 AS PRODUCER OF RECOMBINANT HUMAN INTERFERON ALPHA-2B	2003	Bairamashvili D.I. Vorob'Ev I.I. Gabibov A.G. Demin A.V. Miroshnikov A.I. Mart'Janov V.A. Ponomarenko N.A. Shuster A.M.	RU2258081C1
PLASMID VECTOR PRH15A FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B, BACTERIAL STRAIN ESCHERICHIA COLI BL21 DE3 - PRODUCER OF METHIONINE-FREE INTERFERON ALPHA-2B AND METHOD FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B	2017	Kudling Tatyana Viktorovna Karasev Maksim Mikhajlovich Kolobov Aleksej Aleksandrovich Lisitskaya Veronika Igorevna Romanov Nikolaj Ivanovich Savin Ilya Igorevich Elgina Alena Fedorovna Batarin Vladimir Ilich Gorbunova Irina Nikolaevna Antipova Tatyana Olegovna Denisenko Elizaveta Sergeevna Martyushin Sergej Vasilevich Ishchenko Aleksandr Mitrofanovich	RU2697375C2
RECOMBINANT PLASMID DNA PGGF-8 ENCODING POLYPEPTIDE SHOWING PROPERTIES OF HUMAN GRANULICYTIC COLONY-STIMULATING FACTOR AND THE STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE SHOWING PROPERTIES OF HUMAN GRANULOCYTIC COLONY-STIMULATING FACTOR	1996	Korobko V.G. Shingarova L.N. Petrovskaja L.E. Petrenko L.A. Pustoshilova N.M.	RU2113483C1
RECOMBINANT PLASMID DNA pES6-1 ENCODING HUMAN POLYPEPTIDE INTERFERON BETA-1b AND STRAIN Escherichia coli BDEES6 AS PRODUCER OF HUMAN RECOMBINANT INTERFERON BETA-1b	2004	Bairamashvili D.I. Vorob'Ev I.I. Gabibov A.G. Demin A.V. Miroshnikov A.I. Mart'Janov V.A. Ponomarenko N.A. Shuster A.M.	RU2261913C1
RECOMBINANT PLASMID DNA PSS5 ENCODING SYNTHESIS OF RECOMBINANT HUMAN ALPHA-2B INTERFERON, STRAIN OF ESCHERICHIA COLI SS5 AS PRODUCER OF RECOMBINANT HUMAN ALPHA-2B INTERFERON AND METHOD OF ALPHA-2B INTERFERON PREPARING	1999	Cherepanov P.A. Mikhajlova T.G. Cherepanov P.P. Martinenko Dmitrij Leonidovich Shevchuk Aleksandr Anatol'Evich Fedjukin V.S. Nikolaev T.M. Tolkachev B.B. Sventitskij E.N. Urakov N.N. Kalinin Ju.T. Denisov L.A. Tjagotin Ju.V. Martjushin S.V. Ishchenko A.M. Trofimov A.V. Poljakova E.A. Batarin V.I. Shalaeva O.N. Lisitskaja V.I.	RU2165455C1
RECOMBINANT PLASMID DNA pTrcTREN-IL-13 ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pTrcTREN-IL13 - PRODUCENT OF POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13	2006	Toporova Viktorija Aleksandrovna Petrovskaja Lada Evgen'Evna Krjukova Elena Aleksandrovna Karandashova Inga Vadimovna Shingarova Ljudmila Nikolaevna Vul'Fson Andrej Nikolaevich Kirpichnikov Mikhail Petrovich	RU2333960C1
RECOMBINANT PLASMID PFM-IFN-17 PROVIDING EXPRESSION OF INTERFERON ALPHA-2B OF HUMAN, RECOMBINANT PLASMID PFM-AP, PROVIDING EXPRESSION OF ENZYME METHIONINE-AMINOPEPTIDASE E COLI, BIPLASMID STRAIN ESCHERICHIA COLI FM-IFN-AP (PFM-IFN-17, PFM-AP) - PRODUCER (MET-) OF RECOMBINANT INTERFERON ALPHA -2B OF HUMAN	2016	Markov Ilya Aleksandrovich Markova Elena Alekseevna Gaponyuk Polina Petrovna Markova Inna Nikolaevna	RU2610173C1
RECOMBINANT PLASMID DNA PTRC TE-LEP ENCODING POLYPEPTIDE WITH HUMAN LEPTIN PROPERTY AND STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF POLYPEPTIDE WITH HUMAN LEPTIN PROPERTY	2000	Korobko V.G. Aliev T.K. Varfolomeev S.D. Karpova S.K. Krivtsov V.F. Pankov Ju.A.	RU2185438C2

RU 2 326 168 C1

Authors

Shingarova Ljudmila Nikolaevna

Boldyreva Elena Filippovna

Nekrasova Oksana Vasil'Evna

Goncharova Ol'Ga Vladimirovna

Chugunova Nadezhda Muldabekovna

Chugunov Aleksandr Mikhajlovich

Efanov Jurij Georgievich

Dates

2008-06-10—Published

2006-10-20—Filed