FIELD: molecular biology, genetic engineering, biotechnology. SUBSTANCE: recombinant plasmid DNA pASIF62 is constructed by insertion gene encoding α-2 interferon and inverted gene htpR into vector pQE30. Invention proposes also a method for biosynthesis of α-2 interferon in E. coli cells and the following purification of the end product. Method involves transformation of E. coli cells with plasmid DNA pASIF62 and culturing is carried out to turbidity index 0.4-0.6 OD followed by addition of 0.1-0.3 mM of isopropyl-β-thiogalactoside to medium. Using the invention provides the enhanced yield of α-2 interferon. EFFECT: improved preparing method. 4 cl, 9 dwg
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