FIELD: biotechnology, genetic engineering, medicinal microbiology.
SUBSTANCE: invention proposes recombinant plasmid pAce90 expressing Vibrio cholerae cloned ace gene (accessory cholera enterotoxin) and E. coli strain M15[pREP4]pAce90 (KM 194) as a producer of accessory cholera enterotoxin carrying indicated plasmid. Plasmid is prepared by incorporation of Vibrio cholerae ace gene by sites of Bam HI - Hind III in polylinker of vector plasmid pQE30 in orientation providing the transcription direction under control of T5 promoter. The strain E. coli M15[pREP4]pAce90 (KM 194) is a producer of recombinant protein ace wherein this strain is prepared by transformation of the strain E. coli M15[pREP4] with recombinant plasmid pAce90. Production of ace protein occurs by induction with isopropyl-β-O-thiogalactoside with the yield 7-10% of cellular proteins. Using plasmid pAce90 and the strain E. coli (KM 194) provides accelerating the process for preparing Vibrio cholerae ace protein.
EFFECT: improved preparing method of protein ace, valuable properties of plasmid and strain.
3 cl, 2 dwg, 3 ex
