FIELD: biotechnology, molecular biology, preparative biochemistry.
SUBSTANCE: invention relates to methods for isolation of ribonucleic acids (RNAs) and can be used in molecular-biological investigations. Method involves incubation of biological sample with small-dispersed meshed glass treated with 3.5-7% hydrofluoric acid solution preliminary for 2-6 h in buffer solution containing chaotropic agent, separation of sorbent and sorbent-bound RNA by centrifugation, washing out sorbent and elution of RNAs from sorbent with buffer solution containing 5-50 mM of hydrocarbonate ions (HCO3) and 5-10 mM of EDTA at pH 8.0-10.0. Method provides reducing time process, enhancing RNAs yield and to provide isolation of both low-molecular and high-molecular RNA fractions.
EFFECT: improved isolating method.
3 cl, 5 dwg, 2 tbl, 3 ex
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