FIELD: biotechnology.
SUBSTANCE: following is described: a group of inventions including a lysis mixture for isolating DNA from samples of bacteria, fungi, plants, animals and soil, and a method of isolating DNA from samples of bacteria, fungi, plants, animals and soil. Lysis mixture for DNA extraction is a mixture of CTAB buffer containing 2% CTAB, 1.4 M NaCl, 20 mM EDTA, 100 mM Tris-HCl, pH=8.0, with phenol in a volume ratio of 1–1.5:1, respectively.
EFFECT: invention expands the arsenal of means for isolating DNA from samples of bacteria, fungi, plants, animals and soil.
6 cl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
RU2295569C1 |
| METHOD FOR EXTRACTING DNA FROM SOIL | 2018 |
|
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| METHOD FOR DNA ISOLATION FROM PLANTS, SUITABLE FOR PCR | 2017 |
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RU2672378C1 |
| METHOD OF ISOLATION AND PURIFICATION OF DEOXYRIBONUCLEIC ACIDS | 2008 |
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| METHOD FOR RECOVERY OF NUCLEIC ACIDS | 2014 |
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RU2584346C2 |
| CELL-FREE PROTEIN SYNTHESIS SYSTEM BASED ON STAPHYLOCOCCUS AUREUS CELLS, A METHOD OF PROTEIN SYNTHESIS BASED ON STAPHYLOCOCCUS AUREUS CELLS USING A CELL-FREE PROTEIN SYNTHESIS SYSTEM BASED ON STAPHYLOCOCCUS AUREUS CELLS AND A METHOD OF DETECTING PROTEIN SYNTHESIS INHIBITORS USING IT | 2022 |
|
RU2802080C1 |
| METHOD FOR ISOLATION OF PLASMIDS BURKHOLDERIA PSEUDOMALLEI | 2002 |
|
RU2218401C2 |
| METHOD OF ISOLATION OF DNA FROM MICROORGANISMS AND ANIMAL CELLS SUITED FOR POLYMERASE CHAIN REACTION CARRYING OUT | 1997 |
|
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| METHOD FOR PREPARING YEAST OR GRAM-POSITIVE MICROORGANISMS DENATURATED GENOME DNA ASSOCIATED WITH CELLULAR ENVELOPES | 2006 |
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RU2522828C2 |
