METHOD FOR DNA ISOLATION FROM PLANTS, SUITABLE FOR PCR Russian patent published in 2018 - IPC C12Q1/6806 

FIELD: microbiology.

SUBSTANCE: invention relates to molecular biology, in particular to a method for isolating DNA from plants, suitable for PCR. Sample under study is homogenized in a non-extracting buffer containing TE buffer and polyvinylpyrrolidone (PVP). Next, perform primary lysis using TRITON X-10. Resuspend the pellet in TE buffer with PVP, followed by centrifugation. Remove the supernatant. Stage is repeated 2–3 times. Then a lyse solution containing GuSCN, DTT, EDTA, TRIS-HCI is added to the precipitate, mixed and proteinase K and/or RNase A are added. Next, chloroform is extracted and the DNA is concentrated with isopropanol. After DNA precipitation, the precipitate is washed twice, first with 70 % ethanol, and then with acetone. Dry and elute the DNA in TE buffer. Purity of the isolated DNA free of PCR inhibitors relative to A260/A280 protein is 2.0 ± 0.09.

EFFECT: invention allows to obtain a DNA solution of high quality and a high concentration of predominantly nuclear genome, suitable for laboratory research, including PCR.

1 cl, 4 dwg, 2 ex