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Application

2003131557/13, 2003-10-27

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Start date

2003-10-27

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Actual filing date

2003-10-27

(45)

Published

2004-09-20

(72)

Inventor

Osin A.V.Livanova L.F.Kobkova I.M.Eroshenko G.A.Smirnova N.I.

(73)

Holder

Federal'Noe Gosudarstvennoe Uchrezhdenie Rossijskij Nauchno-Issledovatel'Skij Protivochumnyj Institut

STRAIN OF BACTERIUM VIBRIO CHOLERAE KM-168 AS BASE FOR CONSTRUCTION OF LIVE VACCINE AGAINST DIARRHEA DISEASE CAUSED BY PATHOGENIC STRAINS OF VIBRIO CHOLERAE O139 Russian patent published in 2004 - IPC

Abstract RU 2236455 C1

FIELD: biotechnology, molecular biology.

SUBSTANCE: the strain V. cholerae O130 is obtained by method of induced mutagenesis and the following insertion of recombinant plasmid pIEM3 (Km^rTc^r) with cloned gene of cholerain toxin (ctxB) B-subunit. The strain has no hemolytic train, it doesn't comprise the main virulence genes and avirulent for laboratory animals but exhibits high and stable level in production of choleraic toxin B-subunit (it produces 2.5-3 mcg/ml of B-subunit XT in medium) and protective antigens of cholera pathogens of serum group O130 - antigen O139 and polysaccharide capsule providing formation of antibacterial and antitoxic immunity in cholera.

EFFECT: valuable properties of strain.

7 ex

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RU 2 236 455 C1

Authors

Osin A.V.

Livanova L.F.

Kobkova I.M.

Eroshenko G.A.

Smirnova N.I.

Dates

2004-09-20—Published

2003-10-27—Filed