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2 238 565

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Application

2003106815/13, 2003-03-13

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Start date

2003-03-13

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Actual filing date

2003-03-13

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Published

2004-10-20

(72)

Inventor

Mashnin A.V.Strizhakov A.A.Strizhakova O.M.Novikova M.B.Kurinnov V.V.Tsybanov S.Zh.

(73)

Holder

Vnii Veterinarnoj Virusologii I Mikrobiologii

METHOD FOR PREDICTING OVINE AND CAPRINE VARIOLA DUE TO HISTOCHEMICAL IMMUNOENZYMATIC ASSAY BASED UPON MONOCLONAL ANTIBODIES Russian patent published in 2004 - IPC

Abstract RU 2238565 C1

FIELD: veterinary virology.

SUBSTANCE: the suggested method deals with histochemical immunoenzymatic assay by applying peroxidase conjugate based upon monoclonal antibodies of 010.2 clone. Antibodies of this clone are of specificity to antigenic determinants of polypeptides of 36-40 kD molecular weight in ovine and caprine variola viruses. Moreover, detection of antigens of ovine and caprine variola viruses takes place in cytoplasms of infected cells either of primary or passage cell culture of ovine origin. The suggested method is more simplified, sensitive, specific, it provides reliable detection of antigens of ovine and caprine variola viruses and differentiate them against disease agents with clinically similar picture. This method could be applied at research institutes and veterinary laboratories.

EFFECT: higher accuracy of diagnostics.

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RU 2 238 565 C1

Authors

Mashnin A.V.

Strizhakov A.A.

Strizhakova O.M.

Novikova M.B.

Kurinnov V.V.

Tsybanov S.Zh.

Dates

2004-10-20—Published

2003-03-13—Filed