FIELD: biotechnology.
SUBSTANCE: test system for serodiagnosis of cattle nodulous dermatitis contains a cultural positive antigen of cattle nodulous dermatitis virus of E-95 strain, cultural negative normal antigen, capturing antibodies - specific hyperimmune polyclonal rabbit serum, detecting antibodies -specific hyperimmune polyclonal guinea pig serum antispecies conjugate -immunoglobulins against guinea pig IgG, conjugated with horseradish peroxidase, 0.05M carbonate-bicarbonate buffer, tris-buffer solution, washing buffer solution, blocking buffer solution, buffer for sample and conjugate dilution, ABTS solution and staining stopping solution - 1% solution of SDS.
EFFECT: invention provides creation of a modern, highly specific test system for detection of cattle nodulous dermatitis in virus antigen by solid indirect sandwich ELISA, due to application of E-95 strain when receiving specific reaction components for ELISA.
2 cl, 6 dwg, 4 tbl, 6 ex
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