METHOD OF EXPRESS DIAGNOSTICS OF SHEEP AND GOATS Russian patent published in 2018 - IPC G01N33/53 

FIELD: veterinary.

SUBSTANCE: invention relates to the field of veterinary virology, in particular to a method for diagnosing smallpox in sheep and goats. Method for express diagnostics of the goat and sheep pox virus includes sampling pathological material from the focus of an infectious disease, extraction of bacterial DNA from infectious disease from the obtained samples, identification of the species belonging of the causative agent by polymerase chain reaction with fluorescent recognition of the results in real time using a thermal cycler of the RotorGene type based on the presence / absence of the intersection of the fluorescence curve with the threshold line. As a pathological material, samples from a focus of infectious smallpox disease are used, sampling of pathological material is carried out from all animals, located in the outbreak of an infectious disease, and express diagnostics of the obtained pathological material within 24 hours is performed to identify animals carrying smallpox virus at the initial stage of infection. Results of the polymerase chain reaction are taken into account by the presence or absence of the intersection of the fluorescence curve with the threshold line established at the corresponding level, if the growth of the specific signal is observed, then the sample is considered positive - the pox and sheep pox virus is present, while the values of the control samples are within the limits of normal if the growth of a specific signal is not observed, then the sample is considered negative, so the sheep and goat pox virus is absent and the values of the control samples are also within the normal range.

EFFECT: method makes it possible to expand the functional capabilities of the method for diagnosing smallpox and sheep pox.

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