FIELD: veterinary medicine.
SUBSTANCE: invention relates to veterinary medicine. The method for multiplex analysis of B-lymphocytes of cattle to assess the B-cell link of the immune system is that studies are carried out on 10-well glass slides with two control wells with adsorbed positive control B-cells obtained by separation with latex particles coated with monoclonal antibodies to CD19, and T-cells negative control obtained by separation with latex particles coated with monoclonal antibodies to CD3 from a pool of mononuclear blood of horned cattle. A direct method for immunoperoxidase staining is used with the application of monoclonal antibodies to G9 and C2 conformational and C11 linear epitope of cattle IgM molecules as detecting antibodies of the oligoclonal system that do not compete with each other. Next, azure-eosin is stained for morphological examination of B cells: their shape, cell size, nucleus, nuclear-cytoplasmic ratio, chromatin structure.
EFFECT: invention provides for the development of a productive, highly sensitive and specific method for multiplex research.
1 cl, 12 dwg, 3 ex
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|---|---|---|---|
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|
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| STRAIN OF HYBRID CULTURED MAMMALIAN CELLS MUS MUSCULUS L USED FOR PREPARING OF MONOCLONAL ANTIBODIES TO GLYCOPROTEIN ANTIGEN 70 KDA OF CATTLE PERIPHERAL BLOOD MONONUCLEARS | 1992 |
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| STRAIN 8C12 OF PERMANENT INTER-SPECIES HYBRID LINE OF CELLS OF MOUSE Mus. musculus AND SHEEP Ovis aries - PRODUCENT OF MONOCLONAL ANTIBODIES TO GLYCOPROTEIDAL ANTIGEN OF VIRUS OF CATTLE LEUCOSIS | 2008 |
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SU1389284A1 |
