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2 238 979

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IPC

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Application

2003106436/13, 2003-03-11

(24)

Start date

2003-03-11

(22)

Actual filing date

2003-03-11

(45)

Published

2004-10-27

(72)

Inventor

Bykov V.A.Rebrov L.B.Kondakova N.V.Sakharova V.A.Ripa N.V.Kolkhir V.K.

(73)

Holder

Nauchno-Issledovatel'Skij I Uchebno-Metodicheskij Tsentr Biomeditsinskikh Tekhnologij

METHOD FOR ASSAY OF ANTIRADICAL ACTIVITY OF SUBSTANCES IN VITRO Russian patent published in 2004 - IPC

Abstract RU 2238979 C1

FIELD: biotechnology.

SUBSTANCE: invention can be used for assay of antiradical activity of substances by their ability to interact with radicals OH. Determination of value of constant rate reaction of substance with radicals OH (k_OH) is carried out under conditions of γ-irradiation using p-nitrosodimethylaniline an aqueous solution as a comparison standard. Substances with value k_OH above (4-5) x 10⁹ l/mole x s are selected. Then ratios of equal-damaging doses are determined successively at γ-irradiation of trypsin, DNA and erythrocytes in the presence of selected substance and without it and antiradical activity of analyzed substance is evaluated by comparative analysis. Invention provides carrying out the quantitative evaluation of antiradical activity of biologically active substances (BAS), to compare antiradical and antioxidant effects of BAS and to make reliable conclusions about properties of BAS.

EFFECT: improved assay method.

1 tbl

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RU 2 238 979 C1

Authors

Bykov V.A.

Rebrov L.B.

Kondakova N.V.

Sakharova V.A.

Ripa N.V.

Kolkhir V.K.

Dates

2004-10-27—Published

2003-03-11—Filed