HUMAN MODIFIED TNFα MOLECULE ABLE TO INDUCE FORMATION OF NEUTRALIZING ANTIBODY RAISED TO HUMAN TNFα, DNA ENCODING THEREOF, VECTOR (VARIANTS), METHOD FOR PREPARING, VACCINE AGAINST TNFα (VARIANTS), METHOD FOR TESTING FOR PRESENCE OF TNFα, METHOD FOR TESTING HUMAN BODY FLUID FOR PRESENCE OF TNFα, METHOD FOR DIAGNOSIS, METHOD FOR TREATMENT AND PROPHYLAXIS, MEDICINE FOR TREATMENT Russian patent published in 2004 - IPC

FIELD: biotechnology, genetic engineering, medicine.

SUBSTANCE: human modified TNFα molecule able to elevate amount of neutralizing antibodies raised to human TNFα of wild type after administration of the modified TNFα to a human-recipient is prepared by replacing at least one peptide fragment in human TNFα molecule for at least one peptide comprising immunodomonant epitope for T cell or for creation of truncated form of above indicated molecule comprising immunodominant epitope and one or both flanking regions of human TNFα molecule comprising at least one TNFα epitope for B cell being the replacement results to significant exchange of amino acid sequence in an anterior β-layer in any connective loops and/or in any B', I or D chains in a posterior β-layer. Human modified TNFα or DNA molecules encoding there are used in prescriptions as vaccines against TNFα by choosing with pharmaceutically acceptable substances enhancing the immunogenicity of antibodies, for prophylaxis or treatment of chronic inflammatory diseases, such as rheumatoid arthritis and intestine inflammatory diseases, cancer, cerebrospinal sclerosis, diabetes mellitus, psoriasis, osteoporosis or asthma. Human body fluids are studied for the presence of TNFα in contact with the modified TNFα-containing composition. Invention provides carrying out the development of agents used in treatment of chronic inflammatory diseases. Invention can be used for preparing human modified TNFα molecule able to induce formation of neutralizing antibodies.

EFFECT: improved preparing method, valuable biological and medicinal properties of modified human tumor necrosis factor-α.

37 cl, 33 dwg, 3 tbl, 5 ex