METHODS FOR PRODUCING CELLS EXPRESSING CHIMERIC ANTIGEN RECEPTOR Russian patent published in 2021 - IPC C12N5/02 C12N5/783 A61P35/00 C07K14/725 A61K35/17 

FIELD: biotechnology.

SUBSTANCE: options are proposed of a method for producing a population of immune effector cells expressing a chimeric antigen receptor (hereinafter – CAR) binding to a B-cell antigen. A population of immune effector cells is obtained from the subject having chronic lymphocytic leukemia (hereinafter – CLL). Then, the specified population is brought into the contact with an anti-CD25 antibody or CD25-binding ligand to remove regulatory CD25+ T cells and CD25-expressing CLL cells. Further cells are transduced with nucleic acid encoding CAR to obtain a population of immune effector cells expressing CAR depleted by more than 50% of regulatory CD25+ T cells and CD25-expressing CLL cells. The invention also relates to populations of immune effector cells for treating the subject having hematological cancer, reaction mixture for their production and pharmaceutical composition containing the specified cell population.

EFFECT: invention provides for increased efficiency of transduction in the result of cell depletion of CD25, which can be used for more efficient CART therapy.

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