FIELD: biotechnology.
SUBSTANCE: invention relates to a method of producing a population of cells which express a chimeric antigen receptor (CAR). Said method involves: (i) contacting the cell population with an agent which stimulates the CD3/TCR complex and/or an agent which stimulates a co-stimulating molecule on the surface of the cells; (ii) contacting a population of cells with a nucleic acid molecule encoding a CAR, thereby obtaining a population of cells containing a nucleic acid molecule, and (iii) collecting the cell population for storage or introduction, wherein step (ii) is carried out together with step (i) or not later than 20 hours after the beginning of step (i), and step (iii) is carried out not later than 30 hours after the beginning of step (i).
EFFECT: invention is effective for producing a population of cells which express a chimeric antigen receptor (CAR).
66 cl, 49 dwg, 26 tbl, 15 ex
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Authors
Dates
2024-07-03—Published
2019-08-30—Filed