FIELD: medicine microbiology, in particular technology for reproduction of brucellous bacteriophage.
SUBSTANCE: brucellous bacteriophage Bk2 is reproduced in liquid synthetic nutrient medium. Nutrient medium is used both to provide suspension of growth strain and further to reproduce of bacteriophage. Nutrient medium contains glucose as additional carbon source, amino acids and salts, and additionally 10-2-10-3 M of calcium sulfate. Method of present invention allows the ability to increase final brucellous bacteriophage Bk2 concentration wherein bacteriophage is reproduced by 48-72 h.
EFFECT: brucellous bacteriophage reproduction with increased bacteriophage concentration.
3 ex
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Authors
Dates
2005-04-10—Published
2003-06-16—Filed