FIELD: medicinal microbiology. SUBSTANCE: method involves preparing brucellosis pathogen culture suspension on Marten's broth containing calcium and magnesium sulfate up to concentration 107 CFU/ml. Suspension is incubated at 37 C for 4-6 h and homologous bacteriophage is added to its in the amount providing an infection multiple - 0.01-0.001 (i. e. about 107-106 CFU/ml). Then a mixture is heated at 37 C for 20-30 min, diluted with Marten's broth and incubated at 37 C for 24-36 h. EFFECT: increased yield of bacteriophage, decreased time of process. 3 ex
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Authors
Dates
1999-02-27—Published
1997-10-21—Filed