METHOD FOR QUANTITATIVE DETECTION OF MUCIN Russian patent published in 2005 - IPC

FIELD: biomedicine, laboratory diagnostics.

SUBSTANCE: the quantity of salivary mucin should be detected due to spectrophotometric method by the difference of protein concentration in initial material and supernatant developed after acidic precipitation of mucin. For this purpose two experimental samples should be prepared: the first contains saliva and working reagent, the second - supernatant and working reagent; and standard sample containing aqueous albumin solution at 0.25 g/l concentration and working reagent; and a control sample containing distilled water and working reagent. The latter should be obtained due to mixing the solution of bromine phenol blue at concentration of 1.2 g/l and buffer solution (pH 3.0) containing 320 mM/l citric acid and 160 mM/l sodium phosphate at the ratio of 2:23. The content of each sample should be mixed and incubated for 10 min. One should detect optic density of experimental and standard samples against control one at wave length of 620 nm. Salivary protein concentration and that of supernatant should be calculated in g/l: C = (OD_ex : OD_st)x 0.25, where OD_ex - optic density of experimental sample, OD_st - optic density of standard sample. The method is very simple in its implementation, its conditions provide stability of stained product that enables to simultaneously investigate many samples.

EFFECT: higher efficiency of detection.