PROTEINS CAUSING ALTERNATED IMMUNOGENIC RESPONSE AND METHODS FOR THEIR PREPARING AND USING Russian patent published in 2007 - IPC C12N15/54 C12N15/00 C12N15/11 C12N15/63 C12N1/21 A61K39/02 A61K39/07 

FIELD: biotechnology, immunology, genetic engineering.

SUBSTANCE: invention relates to a variant of protein subtilysine from Bacillus amyloliquiefaciens with change Y217L and comprising T-cellular epitope. This T-cellular epitope of indicated variant comprises one or some amino acid changes chosen from group consisting of residues corresponding to positions: 76, 79 and 122 wherein indicated subtilysine variant has optionally change at one or some following positions: 3, 31, 40, 41, 46, 47, 48, 50, 76, 101, 104, 107, 111, 128, 147, 154, 181, 182, 183, 185, 206, 215, 218, 238, 247, 248, 250, 254, 258 and 262. Also, invention relates to DNA molecules that encode subtilysine variants, host-cells containing this DNA, and to compositions used in skin care and containing indicated subtilysine variants. Invention provides preparing subtilysine variants that elicit reduced immunogenic response in human as compared with the parent subtilysine.

EFFECT: improved and valuable immune properties of protein, improved preparing methods.

31 cl, 25 dwg, 3 tbl, 14 ex