STRAIN OF BACTERIUM BACILLUS SUBTILIS - A PRODUCER OF HYDROLYTIC ENZYME COMPLEX ENRICHED WITH β-GLUCANASE Russian patent published in 1995 - IPC

FIELD: biotechnology, agriculture, food industry, brewery. SUBSTANCE: method involves multiple sowing the strain B. subtilis ВКПМ-13-5165, isolation and purification of colonies on the media containing lichenan as a nutrition source, and the strain B. subtilis ВКПМ B-5790 is obtained. The strain B. subtilis is cultured on the nutrient medium containing brewery dregs, БВК, 1% KH₂PO₄,, pH 6.8-7.2, cultivation temperature is 33-37 C, cultivation time is 266-32 hr. Cultural fluid has the following enzymatic activities: 1,3-1,4-β-glucanase, 1,3-1,4-b-glucosidase, b-endoglucanase, 1,4-mannanase, xylanase (total cytolytic activity) 20-30 U/ml a-glucoamylase, neutral, alkaline and acid proteases. EFFECT: isolation of bacterial strain indicated above.