MIKANOLID AND DIHYDROMIKANOLID AS INHIBITORS OF DNA-POLYMERASES Russian patent published in 2005 - IPC

FIELD: biochemistry, medicine, pharmacy.

SUBSTANCE: leaves of Mikania micrantha, Mikania scandens and Mikania cordata are milled and dries and a solvent - toluene, ethyl acetate, their mixture, mixture of toluene with acetone, mixture of ethyl acetate with heptane or mixture of heptane with acetone is added in the ratio (7:3)-(3:7), mixture is filtered and concentrated up to 2.5-10% as measure for dry extract. Then extract is contacted with a mixture containing 10-50% of methanol or ethanol in water, an aqueous-alcoholic phase is washed out with n-hexane or heptane and alcohol is removed. An aqueous phase is extracted with ethyl acetate, prepared phase is dried, solvents are evaporated and dry extracted is purified. Dry extract prepared from leaves is dissolved in ethyl acetate and hydrogenated at 10-35°C in the presence of hydrogenation catalyst under pressure 1-2 atm. After hydrogenation dihydromikanolid is crystallized. Mikanolid and dihydromikanolid are used for treatment proliferative diseases and parasitic diseases. Also, invention relates to a medicinal agent containing above indicated vegetable extract. Invention provides realization of indicated designation.

EFFECT: valuable properties of agents.

12 cl, 3 tbl, 3 ex