FIELD: cryobiology, cryomedicine.
SUBSTANCE: method involves addition cryoprotective medium to sperm and its freezing with liquid nitrogen vapors at super high rates of freezing. Also, invention relates to composition of cryoprotective medium comprising glycerol ester, tris-buffer, EDTA, lecithin, kanamycin sulfate and water in special containers. Method provides significant enhancing viability and motility of spermatozoons and to increase frequency of pregnancy occurring after impregnation with sperm cryopreserved by proposed method.
EFFECT: improved cryopreserving method.
2 cl, 3 tbl, 3 ex
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