METHOD FOR CATTLE OOCYTE FREEZING Russian patent published in 2019 - IPC A01N1/02 C12N5/07 

Abstract RU 2707252 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. This method for vitrification of oocytes of mammals can be successfully applied to agricultural animals, in particular for cattle. Oocytes from cattle are recovered by aspiration from antral follicles, conducting morphological selection and washing with physiologic saline. It is then frozen in series in three vitrification solutions containing a combination of cryoprotectors and excipients, after which content with oocytes is placed in a cryotube partially submerged into liquid nitrogen, and after tightening the cap, the cryotube is immersed into a container with liquid nitrogen and then into a Dewar vessel. Disclosed method of freezing oocytes of cattle is less toxic due to reduction of cryoprotectors, invention allows protect a considerable part of oocytes from damages.

EFFECT: as a result of this vitrification procedure efficiency of revitalization of oocytes after thawing in more than 80 % of cases is achieved.

7 cl, 9 dwg, 2 tbl

Similar patents RU2707252C1

Title Year Author Number
METHOD OF VITRIFICATION OF OVARIAL TISSUE 2018
  • Kiseleva Marina Viktorovna
  • Malinova Irina Vadimovna
  • Komarova Elena Vladimirovna
  • Kaprin Andrej Dmitrievich
RU2678106C2
METHOD FOR OPTIMIZING CRYOPRESERVATION OF OVARIAN TISSUE FOR LONG-TERM STORAGE 2022
  • Komlichenko Eduard Vladimirovich
  • Pervunina Tatyana Mikhajlovna
  • Ulrikh Elena Aleksandrovna
  • Govorov Igor Evgenevich
  • Dikareva Elena Leontevna
  • Dinikina Yuliya Valerevna
  • Kokhreidze Nadezhda Anatolevna
  • Belogurova Margarita Borisovna
  • Dejnega Viktor Yurevich
  • Vasyutina Marina Lvovna
  • Tsinzerling Vsevolod Aleksandrovich
RU2794963C1
UNGULATE EMBRYO CRYOPRESERVATION 2016
  • Sanches, Bruno Valente
RU2730597C2
SYSTEM AND DEVICE (VERSIONS) FOR CRYOPRESERVATION OF GROUP OOCYTES AND EMBRYOS OF MAMMALS BY VITRIFICATION WHEN USED AS HOLLOW FIBRE 2014
  • Malenko Galina Petrovna
  • Kornienko Ekaterina Valerevna
  • Kosovskij Gleb Jurevich
RU2584584C2
METHOD FOR STIMULATING THE GROWTH OF FOLLICLES IN THE PROGRAM OF IN VITRO FERTILIZATION IN PATIENTS WITH INFERTILITY AND "POOR RESPONSE TO STIMULATION" 2017
  • Mazurov Dmitrij Olegovich
RU2665967C1
METHOD FOR CULTURING OF OVARIAN FOLLICLES IN VITRO 2001
  • Kortvrind Rita
  • Smitz Jogan
RU2286384C2
METHOD OF PREDICTING EFFECTIVENESS OF ASSISTED REPRODUCTIVE TECHNOLOGY PROGRAMS BASED ON NEURAL NETWORKS 2022
  • Portnov Igor Grigorevich
  • Lisovskaia Tatiana Valentinovna
  • Rezaikin Aleksei Vasilevich
  • Maiasina Elena Nikolaevna
  • Salimov Daniil Fratovi
  • Kogan Igor Iurevich
  • Ivanov Andrei Valentinovich
  • Gzgzian Aleksandr Mkrtichevich
  • Lisovskii Stanislav Mikhailovich
  • Askerov Roman Askerovich
RU2809429C1
CRYOPRESERVING SOLUTION NOT CONTAINING DMSO AND ITS PRODUCTION METHOD AND USE 2020
  • Yan, Tsze
  • Tsyao, Tsze
  • Yan, Liin
  • Li, Zhun
  • Van, Tszyantszyun
  • Tszin, Shenlin
  • Lv, Tszyanyun
RU2785227C1
METHOD FOR EXTRACORPOREAL CULTIVATING BOVINE OOCYTES 2009
  • Lebedeva Irina Jur'Evna
RU2410063C1
METHOD FOR CRYOPRESERVATION OF PLASMA MICROVESICLES 2020
  • Sokolov Dmitrij Igorevich
  • Markova Kseniya Lvovna
  • Selkov Sergej Alekseevich
RU2746950C1

RU 2 707 252 C1

Authors

Abakushina Elena Vyacheslavovna

Gelm Yuliya Vitalevna

Mitsenyk Anastasiya Sergeevna

Dates

2019-11-25Published

2018-08-02Filed