FIELD: veterinary virology, biotechnology.
SUBSTANCE: new industrial strain of rotavirus serotypes G8P7 in cattle is obtained by successive passages in 3-day old culture of MDBK cells. The strain is deposited in strains microorganism collection in VGNKI at registration number "TE87"-DEP. Virus of the strain № "TE87"-DEP multiplies good in cell cultures MDBK and Marc-145 and for 72-96 h it accumulates with the titers 4.5-7.5 lg TCD50/ml. The strain is stable and retains indices in successive passage in sensitive biological systems for 9-10 passages (observation period). The strain № "TE87"-DEP elicits the broad antigen spectrum of action and high biological activity and can be used for preparing effective diagnostic preparations.
EFFECT: valuable properties of strain.
3 dwg, 3 tbl, 5 ex
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Authors
Dates
2005-08-20—Published
2003-12-24—Filed