METHOD FOR DETERMINING NARCOTIC SUBSTANCES OF OPIATE GROUP IN CADAVER BLOOD AND URINE IN QUANTITATIVE AND QUALITATIVE WAY Russian patent published in 2005 - IPC

FIELD: forensic medicine.

SUBSTANCE: method involves applying immunoenzyme analysis using worksheet bearing immobilized narcotic substance antigen and introducing medium under study into worksheet cells. The medium is diluted in advance X times with phosphate buffer having pH=6.5. Antibodies bound to enzyme label are added to morphine and incubated at 18-20°C. The immunoenzyme analysis results are recorded in spectrophotometric way. No opiates case is detected from optical density (OD) of diluted medium under study (OD_us) being equal to optical density of negative control solution (OD_neg.cs). Permissible error value is not to be greater than 30% of OD_neg.cs value. Opiate availability is established to be in diagnostically significant concentration of 300 ng/ml from OD_us being in the range of OD_{positive cs}≥OD_us>0. OD_us=0 being the case, finite dilution is carried out until OD_us becomes unequal to zero. Then, narcotic substances of opiate group are to be quantitatively determined by using a formula.

EFFECT: simplified and accelerated method.

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