METHOD FOR DIAGNOSING NARCOTIC DRUG CONSUMPTION Russian patent published in 2007 - IPC G01N33/547 G01N33/94 

FIELD: medicine.

SUBSTANCE: method involves applying solid phase immune-enzyme assay for determining antibodies availability in blood serum. The analyzed sample is incubated with hapten : macromolecular carrier immobilized on solid phase taken as conjugate. The so formed immune complex is next incubated with conjugate of antibodies against human IgM and IgG marked with enzyme. Specific antibodies level is determined by taking difference from optical density of the sample under analysis and the like reference sample values obtained when drugs are available and not available in blood serum. Drug consumption is considered to be the case, if antibody levels in the sample under analysis are equal to or greater than in reference sample with the difference that antibodies to endogenous bioregulators are determined in blood serum with conjugate having hapten belonging to opioid peptide group from β-endomorphine, dermorphine, enkephalins row or biogenous amines from catecholamine, serotonin, histamine row. Poly(4-nitrophenyl)acrylate is taken as the macromolecular carrier. Hapten : macromolecular carrier ration is equal to (10-15):1 in mole%.

EFFECT: high accuracy at early stage of drug consumption.