FIELD: immunology, proteins.
SUBSTANCE: invention proposes a method for preparing affinity surfaces based on chemically cross-linked staphylococcus protein A. Method involves applying a layer of staphylococcus protein A on hydrophobic surface followed by chemical cross-linkage of protein A. Also, method involves additional applying specific antibodies on the prepared affinity surface with a layer of chemically cross-linked staphylococcus protein A. This method provides preparing the affinity surface eliciting the enhanced strength that can be separated from a backing if necessary and with retaining its integrity. Also, the flat and uniform surface of layer of crossed-linked staphylococcus protein A provides low level of interferences in carrying out study by different methods of microscopy. Invention can be used in investigation of biological objects by different methods, among them by method of atomic-force microscopy. Invention can be used in immunological researches of biological objects.
EFFECT: improved preparing method.
3 dwg, 3 ex
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