FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Disclosed is a test kit for detecting cholera toxin in a culture medium. Test kit contains a weight of AKI, a conjugate of monoclonal antibodies specific to B-subunit of cholera toxic isotype G1 with horseradish peroxidase, reagents for conjugate dilution, reagents for preparation of substrate mixture and dipstick. Dipstick is a strip of material resistant to aqueous solutions with a fixed on it membrane fragment sensitized with cholera toxin-specific monoclonal antibodies, and has on the surface additionally immobilized internal positive reaction control.
EFFECT: invention provides a highly specific, biologically less hazardous, fast and highly effective detection of cholera toxin in a culture medium with reduced labour costs and consumption of reactants.
1 cl, 5 ex, 2 tbl, 4 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD AND SET FOR DETERMINATION OF CHOLERA TOXIN PRODUCTION AND DIFFERENTIATION OF EPIDEMIOLOGICALLY SIGNIFICANT STRAINS OF CHOLERA VIBRIOS OF CLASSICAL AND EL TOR BIOVARS | 2016 |
|
RU2611359C1 |
| COMPLEX GENE- AND IMMUNODIAGNOSTIC TEST-SYSTEM FOR IDENTIFICATION OF CHOLERA VIBRIONS OF O1 AND O139 SEROGROUPS AND ESTIMATION OF THEIR VIRULENCE | 2009 |
|
RU2404257C1 |
| CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS HT 3E5 - PRODUCER OF MONOCLONAL ANTIBODIES ISOTYPE G 2A TO B- SUBUNIT OF CHOLERA TOXIN | 2015 |
|
RU2590587C1 |
| HYBRID STRAIN OF CULTURED CELLS OF ANIMALS MUS MUSCULUS -T 2E5 - PRODUCER OF MONOCLONAL ANTIBODIES ISOTYPE G 1 TO B SUBUNIT OF CHOLERA TOXIN | 2015 |
|
RU2583306C1 |
| AVIRULENT STRAIN OF Vibrio cholerae KM 263 BACTERIA OF SEROVAR INABA OF BIOVAR ELTOR - PRODUCER OF PROTECTIVE O1 ANTIGEN | 2010 |
|
RU2425867C1 |
| STRAIN OF BACTERIA VIBRIO CHOLERAE - PRODUCER OF CHOLERAIC TOXIN OF II TYPE | 2007 |
|
RU2326941C1 |
| AVIRULENT STRAIN OF Vibrio cholerae KM 262 BACTERIA OF SEROVAR OGAWA OF BIOVAR ELTOR - PRODUCER OF PROTECTIVE O1 ANTIGEN | 2010 |
|
RU2425868C1 |
| STRAIN OF BACTERIUM VIBRIO CHOLERAE KM 206 OF CLASSIC BIOVAR SEROVAR OGAVA AS PRODUCER OF PROTECTIVE ANTIGENS | 2002 |
|
RU2222594C1 |
| STRAIN OF BACTERIUM VIBRIO CHOLERAE KM 207 OF CLASSIC BIOVAR SEROVAR INABA AS PRODUCER OF PROTECTIVE ANTIGENS | 2002 |
|
RU2222595C1 |
| METHOD FOR PRODUCING A DIAGNOSTICUM FOR DETECTING A TOXIN OF A CHOLERA VIBRIO RECOVERED FROM ENVIRONMENTAL OBJECTS | 2019 |
|
RU2703282C1 |
