FIELD: biotechnology, microbiology, organic chemistry.
SUBSTANCE: invention relates to a method for preparing organic acids, in particular, citric acid. Method involves isolation of calcium citrate and acid stable amylolytic enzymes from the solution cultural fluid after fermentation with fungus Aspergillus niger. The isolation process is carried out at temperature 10-50°C and pH 3.2-5.9. Method provides increasing yield of calcium citrate and complex of acid stable amylolytic enzymes: α-amylase and glucoamylase.
EFFECT: improved preparing method.
1 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING CITRIC ACID AND ACID-STABLE AMYLOLYTIC ENZYMES COMPLEX | 2005 |
|
RU2294371C2 |
| METHOD FOR PREPARING CITRIC ACID | 2001 |
|
RU2233882C2 |
| METHOD OF PRODUCING CALCIUM CITRATE | 2018 |
|
RU2703719C1 |
| METHOD FOR PREPARING CITRIC ACID | 2001 |
|
RU2215036C2 |
| FOOD SUPPLEMENT FOR HYDROLYSIS OF PLANT RAW MATERIALS | 2005 |
|
RU2294368C1 |
| METHOD FOR PRODUCTION OF CITRIC ACID, ALPHA-AMYLASE AND GLUCOAMYLASE | 2004 |
|
RU2266960C2 |
| METHOD OF CITRIC ACID PRODUCING | 2000 |
|
RU2186850C2 |
| STRAIN OF MYCELIAL FUNGUS ASPERGILLUS AWAMORI AS PRODUCER OF GLUCOAMYLASE | 2000 |
|
RU2196821C2 |
| METHOD OF COMPLEX PROCESSING GRAIN RAW MATERIAL FOR ALCOHOL AND FEED PRODUCTS ENRICHED IN LYSINE | 2009 |
|
RU2396007C1 |
| METHOD FOR PREPARATION OF CITRIC ACID, ?-AMILASE AND GLUCOAMYLASE | 2007 |
|
RU2366712C2 |
