FIELD: genetic engineering, proteins, medicine, pharmacy.
SUBSTANCE: invention relates to a method for preparing a fused protein representing immunoglobulin Fc-fragment and interferon-alpha and can be used in treatment of hepatitis. Method involves construction of a fused protein comprising immunoglobulin Fc-fragment prepared from Ig G1 or Ig G3 in direction from N-end to C-end and the end protein comprising at least one interferon-alpha. Fc-fragment and the end protein are joined directly or by a polypeptide bridge. The fused protein is used for preparing a pharmaceutical composition used in treatment of liver diseases and in a method for targeting interferon-alpha into liver tissues. Invention provides preparing the fused protein eliciting with biological activity of interferon-alpha providing its concentrating in liver and showing enhanced solubility, prolonged half-time life in serum blood and enhanced binding with specific receptors.
EFFECT: improved targeting method, valuable biological properties of fused protein.
10 cl, 5 dwg, 9 ex
| Title | Year | Author | Number |
|---|---|---|---|
| HOMODIMERIC FUSED PROTEIN WITH ACTIVITY OF ANGIOGENESIS INHIBITOR (VARIANTS), DNA MOLECULE ENCODING HOMODIMERIC FUSED PROTEIN, VECTOR FOR EXPRESSION OF HOMODIMERIC FUSED PROTEIN, METHOD FOR TRANSFECTION OF MAMMALIAN CELL AND METHOD FOR PREPARING HOMODIMERIC FUSED PROTEIN | 1999 |
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