FIELD: biotechnology.
SUBSTANCE: invention relates to mutant carbamoyl-phosphate synthase from Escherichia coli wherein amino acid sequence in 947-951-positions of natural carbamoyl-phosphate synthase in replaced with any amino acid sequences from SEQ ID NO:1- SEQ ID NO:9. Similar mutant carbamoyl-phosphate synthase from Escherichia coli containing any deletions, insertion, substitutions, or additions of one or more amino acids in one or more positions excluding 947-951-positions is described. Also disclosed are DNA fragment encoding aforementioned mutant carbamoyl-phosphate synthases, as well as method for production of carbamoyl-phosphate derivatives using strain Escherichia coli modified with said DNA fragment. Further invention relates to various strains Escherichia coli, for instance: strain Escherichia coli 311 that is producer of orotic acid; strain Escherichia coli 333 that is producer of L-arginine and citrulline; strain Escherichia coli 374 that is producer of citrulline.
EFFECT: method for production of carbamoyl-phosphate derivatives such as L-arginine, citrulline, pyrimidine derivatives in increased amounts as compared with natural strains Escherichia coli.
10 cl, 2 dwg, 5 tbl, 4 ex
