FIELD: biotechnology, biochemistry, molecular biology, microbiology, amino acids. SUBSTANCE: L-arginine is prepared by culturing microorganism of genus Escherichia transformed with DNA fragment encoding mutant N-acetylglutamate synthase. Mutant N-acetylglutamate synthase is the synthase wherein sequence of amino acids corresponding to positions from 15 to 19 in natural N-acetylglutamate synthase is replaced by any of sequence of amino acids at numbers 1-4 (SEQ ID NOS: 1-4) or the synthase showing indicated properties and containing additionally deletions, substitutions, insertions or additions of one or some amino acids by one multiple positions except for positions from 15 to 19. Strains of Escherichia coli 237/pMADS11, E. coli 237/pMADS12 or E. coli 237/pMADS13 can be used strain-producers of L-arginine. EFFECT: enhanced yield of arginine. 9 cl, 3 tbl, 1 dwg, 1 ex
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