FIELD: biotechnology, pharmacy.
SUBSTANCE: statin compounds from fermentation solution are purified by extraction and crystallization. Fermentation broth is subjected for preliminary treatment that involves preliminary alkaline treatment followed by extraction of nonpolar impurities. After preliminary treatment the statin compound is extracted into hydrophobic solvent that is separated after extraction. Then solution of hydrophobic organic solvent is concentrated and washed out, if necessary, with a base-containing aqueous solution. Then extracted statin compound is purified by crystallization. Invention provides isolating statins from fermentation broth with high degree of effectiveness and under pharmaceutically acceptable level of purity.
EFFECT: improved purifying method.
28 cl, 8 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR PREPARING STATIN SODIUM SALTS | 2000 |
|
RU2246481C2 |
INDUSTRIAL METHOD OF PRODUCING COMPACTIN | 2013 |
|
RU2585233C2 |
MICROBIAL METHOD FOR PRAVASTATIN PRODUCTION | 2000 |
|
RU2252258C2 |
METHOD OF PRODUCING COMPACTIN | 2013 |
|
RU2585234C2 |
HYDROXYLATION OF COMPACTIN TO PRAVASTATIN USING MICROMONOSPORA | 2000 |
|
RU2235780C2 |
METHOD FOR ISOLATION AND PURIFICATION OF HMG-COA-REDUCTASE INHIBITOR | 1999 |
|
RU2235130C2 |
FUNGUS STRAIN ASPERGILLUS TERREUS 44-62 AS PRODUCER OF LOVASTATIN, INDUSTRIAL METHOD FOR ISOLATION OF LOVASTATIN AND METHOD FOR LACTONINIZATION OF STATINS | 2003 |
|
RU2261901C2 |
METHOD OF ISOLATION OF LOVASTATIN | 1994 |
|
RU2114912C1 |
METHOD FOR PURIFYING PRAVASTATIN | 2001 |
|
RU2260582C2 |
METHOD OF PREPARING CLAVULANIC ACID OR ITS PHARMACEUTICALLY ACCEPTABLE SALTS OR ESTERS | 1995 |
|
RU2088586C1 |
Authors
Dates
2005-12-10—Published
2001-01-25—Filed