FIELD: biotechnology, microbiology, medicine, pharmacy.
SUBSTANCE: invention relates to a method for preparing and concentrating cytotoxic substances, in particular, epotilones and their using for preparing pharmaceutical compositions used in treatment of proliferative diseases. Method for concentrating epotilones involves addition of a complex-forming component taken among the group of cyclodextrins to an aqueous cultural medium used in growing the producer, - Sorangium cellulosum. Also, invention describes a cultural medium used for growing S. cellulosum that contains the abovementioned complex-forming component and a method for preparing epotilones by using this medium. Invention represents a method for separating prepared epotilones being primarily epotilones A and B by method of reverse column chromatography and a (lower)-alkylcyanide containing eluent. Invention describes crystalline form of epotilone B and its using in pharmaceutical composition for treatment of proliferative diseases and a method for treatment of these diseases. Invention provides preparing epotilones in the industrial scale.
EFFECT: improved preparing method.
16 cl, 6 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
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| APPLYING EPOTILONES FOR CANCER TREATMENT | 1999 |
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| WATER-INSOLUBLE CYCLODEXTRINE POLYCONDENSATE: APPLICATION AS A CAPTURING AGENT | 2013 |
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