METHOD FOR INTRASPECIES DIFFERENTIATION OF Vibrio cholerae O139 Russian patent published in 2006 - IPC C12Q1/04 C12R1/63 C12R1/92 

FIELD: virology, medicinal microbiology.

SUBSTANCE: method involves using phage-sensitive strains of Vibrio cholerae eltor KM-199 and Vibrio cholerae O139 KM-152 as test-strains, and differentiation is carried out for two steps. At the first step Vibrio cholerae eltor KM-199 is used as the strain-indicator and this strain is incubated with analyzed culture for 20-24 h. Then prepared mixture of microorganisms is heated at temperature 55-57°C for 40-60 min and inoculated on lawn with the strain-indicator Vibrio cholerae eltor KM-199 that is inoculated preliminary in layer 0.7% of agar on plate with 1.5% Marten's agar. After culturing for 24 h at 37°C phagolysis is recorded. The second differentiation step is carried out by using phage obtained from lysogenic strain of analyzed culture by its applying on the strain-indicator Vibrio cholerae O139 KM-152 that is used for detection of filamentous phages only by carrying out the direct phagotyping. Using the proposed method allows carrying out phagotyping with high precision and obtaining additional indices of choleraic vibrios isolating from clinical material. Invention can be used in laboratory diagnosis of cholera.

EFFECT: improved and valuable method for differentiation.

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